Simple DNA Probe Assays Based on Particle Agglutination
نویسندگان
چکیده
منابع مشابه
Simple DNA probe assays based on particle agglutination.
I describe the use of latex agglutination as a simple readout for DNA hybridization assays. Latex agglutina-tion is a well-known readout for antibody-based diagnos-tics (1, 2) and uses " sandwich " assay chemistry for which analogous DNA assays are well known (3, 4). Latex agglutination is extremely simple and chemically robust, and latex particles have been used as a label in DNA hybridization...
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Latex beads (0.8-micron diameter; Difco Laboratories) were coated with fibronectin, fibrinogen, collagen type I, or denatured collagen (gelatin) and evaluated in a particle agglutination assay (PAA) for the rapid detection of fibronectin, fibrinogen, or collagen binding to Staphylococcus aureus. These assays were compared with a commercial test for detecting the binding of fibrinogen and immuno...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1998
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/44.4.876